{"id":27980,"date":"2023-11-29T21:01:12","date_gmt":"2023-11-29T20:01:12","guid":{"rendered":"https:\/\/biocore-diagnostics.de\/?post_type=product&#038;p=27980"},"modified":"2023-11-29T21:05:27","modified_gmt":"2023-11-29T20:05:27","slug":"lenticas9-egfp","status":"publish","type":"product","link":"https:\/\/biocore-diagnostics.de\/de\/produkt\/lenticas9-egfp\/","title":{"rendered":"LENTICAS9- EGFP"},"content":{"rendered":"<p>. LentiCas9-EGFP Information:<br \/>\n. Function Mammal Editing plasmids<br \/>\n. Resistance Amp<br \/>\n. Screen Zeo<br \/>\n. Strain Stbl3<br \/>\n. Culture temperature 37degrees centigrade<br \/>\n. Promoter: EF-1 alpha core promoter<br \/>\n. Copier: Ori, F1 ori, SV40 ori<br \/>\n. Terminator: WPRE<br \/>\n. Plasmid classification: mammalian cell plasmid; mammalian editing plasmid; mammalian Cas9 plasmid<br \/>\n. Plasmid size: 13177 BP<br \/>\n. Plasmid tags: C-nucleoplasmin NLS, C-FLAG, C-EGFP<br \/>\n. Prokaryotic resistance: ampicillin Amp<br \/>\n. Screening Marker: Bleomycin Zeo<br \/>\n. Cloning strain: Escherichia coli Stbl3<br \/>\n. Culture conditions: 37 C, aerobic, LB<br \/>\n. Expressing host: mammalian cells such as 293T<br \/>\n. LentiCas9-EGFP Description<br \/>\n. Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.<br \/>\n. LentiCas9-EGFP Reference<br \/>\n. Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Packing 2ug<\/p>\n","protected":false},"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[15,418,419],"product_tag":[],"class_list":{"0":"post-27980","1":"product","2":"type-product","3":"status-publish","5":"product_cat-all","6":"product_cat-lifescience-markt","7":"product_cat-plasmid","10":"instock","11":"taxable","12":"shipping-taxable","13":"product-type-simple","14":"product-grid-view"},"acf":[],"_links":{"self":[{"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/product\/27980","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/comments?post=27980"}],"wp:attachment":[{"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/media?parent=27980"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/product_brand?post=27980"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/product_cat?post=27980"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/biocore-diagnostics.de\/de\/wp-json\/wp\/v2\/product_tag?post=27980"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}