Description

. Search name: Lenti dcas VP64-Blast,Plasmid Lenti dcas VP64-Blast,Lenti dcas VP64-Blast vector
. Lenti dcas VP64-Blast Information
. Plasmid type: CRISPR/Cas9 vector; gene knockout vector; mammalian vector
. High copy / low copy: high copy
. Cloning method: BsiWI, EcoRI
. Promoter: EF1
. Carrier size: 14085 BP
. 5’sequencing primers and sequences: GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
. 3’sequencing primers and sequences: cacatagcgtaaaaggagcaacatag
. Carrier label: –
. Vector resistance: ampicillin (Ampicilin)
. Screening markers: Blasticidin
. Cloned strain: Stbl3
. Host cells (lines): mammalian cells
. Note: Mutation D10A and N863A in Cas9
. Stability: stable expression
. Composition / inducible type: composition
. Virus / non virus: slow disease
. Use:CRISPR-CAS27-sgRNA plasmid