Description

ViroReal® Kit PEDV & SDCV is a real-time PCR kit for detection of RNA of both porcine epidemic diarrhea virus (PEDV) and swine delta coronavirus (SDCV, also called porcine delta coronavirus PDCoV) using one-step reverse transcription real-time PCR. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of PEDV RNA and SDCV RNA isolated from feces of acutely affected pigs (e.g. with the QIAamp Viral RNA Mini Kit, Qiagen).