Description

. Form liquid
. Alternative Name ATP5H, ATP5JD, ATPase subunit d, ATPQ
. Purification Immunogen affinity purified
. Purity 95% as determined by SDS-PAGE
. Host Rabbit
. Isotype IgG
. Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

. Background/ FUNCTION
. Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
. Immunogen ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Specificity Human, Mouse
. Tested Application ELISA, WB, IHC
. Recommended dilution WB: 1:500-1:2000; IHC: 1:50-1:500
. IHC use Immunohistochemistry of paraffin-embedded human kidney using PAab10033(ATP5H antibody) at dilution of 1:100
. WB use
. A549 cells were subjected to SDS PAGE followed by western blot with PAab10033(ATP5H antibody) at dilution of 1:1000
. Protein Information
. Gene ID 10476
. Uniprot ID O75947
. Calculated MW 19-22 kDa
. Research Area Signal Transduction, Metabolism