Description

BactoReal® Kit Brachyspira pilosicoli is a real-time PCR assay for detection of B. pilosicoli DNA. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of B. pilosicoli from samples purified from faecal samples, or biopsies of the intestinal mucosa. Brachyspira pilosicoli DNA can be recovered efficiently from faecal samples using the QIAamp DNA Stool Mini Kit, and from biopsies using the QIAamp DNA Mini Kit extraction methods, for example.