BactoReal® Kit Clostridium difficile is a real-time PCR assay for detection of DNA of Clostridium difficile toxin Aand B. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of toxigenic Clostridium difficile from samples purified from stool or other sample material (e.g. with the QIAamp DNA Stool Mini Kit).
BactoReal® Kit Clostridium difficile allows the rapid and sensitive detection of Clostridium difficile. This test is performed in a multiplex real-time PCR format to enable differentiation between Clostridium difficile toxin A from toxin B. In VIC/HEX channel the toxin A (tcdA) gene of Clostridium difficile is detected. In FAM channel the toxin B (tcdB) gene of Clostridium difficile is detected.
An internal positive control system for detection in Cy5 channel (667 nm) excludes false-negative interpretation of results due to inhibition of real-time PCR (see 8. Interpretation of PCR-data).
When using PCR-platforms not validated by ingenetix, an evaluation of the multiplex-PCR is recommended.
Please be aware that some PCR-platforms have to be calibrated with the corresponding dye before performing multiplex-PCR.