BactoReal® Kit Mycoplasma gallisepticum is a real-time PCR assay for detection of M. gallisepticum DNA. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Applied Biosystems),
LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of M. gallisepticum from samples purified from faecal samples, or swabs of tissues, exudates, diluted tissue homogenates, aspirates from the infraorbital sinuses or joint cavities, as well as from material from egg yolk or embryos. Mycoplasma gallisepticum DNA can be recovered efficiently from faecal samples using the QIAamp DNA Stool Mini Kit, and from swabs using the QIAamp DNA Mini Kit extraction methods, for example. Mycoplasma imitans is not detected by this test.