ViroReal® Kit PRRS Virus EU & NA 1.1 is a real-time PCR kit for detection of RNA of both viral lineages PRRSV EU (including Lena strain) and PRRSV North America (NA) using one-step reverse transcription real-time PCR. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of RNA of PRRS virus from samples purified from blood, saliva, semen, faeces, nasal secretions, milk and tissue samples of acutely fallen ill pigs or of weak-born piglets (e.g. with the QIAamp Viral RNA Mini Kit, Qiagen).
The new version 1.1 of the kit has been adapted to currently circulating PRRSV EU-type viruses to allow
improved detection of these variants.