ViroReal® Kit Rabies Virus is a real-time PCR assay for detection of RNA of the classical rabies virus (RABV genotype 1, serotype 1, RABV-GT-1) using one-step reverse transcription real-time PCR. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Applied Biosystems), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This kit allows the rapid and sensitive detection of RNA of rabies virus from samples purified from tissues (e.g. brain, etc), saliva, urine and cerebrospinal fluid. Serial sampling is recommended. Rabies virus RNA can be recovered efficiently from liquid samples using the QIAamp Viral RNA Mini Kit (Qiagen) or from tissue samples using RNeasy kit (Qiagen) extraction methods, for example.