BactoReal® Kit American Foulbrood 1.1 is a real-time PCR assay for detection of Paenibacillus larvae DNA. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument ((Thermo Fisher Scientific), LightCycler® 480 (Roche), and for Mx3005P® (Agilent) but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of Paenibacillus larvae from samples purified from swabs, tissues, etc. (e.g. with the QIAamp DNA Mini Kit).