Description

BactoReal® Kit Brachyspira hyo/pilo is a real-time PCR assay for detection of B. hyodysenteriae and B. pilosicoli DNA. Other Brachyspira species are not detected. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® (Agilent) but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive screening for DNA of B. hyodysenteriae and B. pilosicoli from samples purified from faecal samples, or biopsies of the intestinal mucosa. Brachyspira DNA can be recovered efficiently from faecal samples using the QIAamp DNA Stool Mini Kit, and from biopsies using the QIAamp DNA Mini Kit extraction methods, for example.