BactoReal® Kit Mycoplasma gallisepticum & synoviae is a multiplex real-time PCR assay for detection and
differentiation of DNA of Mycoplasma gallisepticum and Mycoplasma synoviae. This test was developed and
validated for the ABI PRISM® 7500 (Fast) instrument (Life Technologies), LightCycler® 480 (Roche) and
Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. The test facilitates the rapid and sensitive detection of DNA of M. gallisepticum and M. synoviae from samples purified from faecal samples, or swabs of tissues, exudates, diluted tissue homogenates, aspirates from the infraorbital sinuses or joint cavities, as well as from material from egg yolk or embryos. Sample pools with up to 20 samples per pool can be analyzed. Mycoplasma DNA can be recovered efficiently from faecal samples using the QIAamp DNA Stool Mini Kit, and from swabs using the QIAamp DNA Mini Kit extraction methods, for example.