Description

. LentiCas9-EGFP Information:
. Function Mammal Editing plasmids
. Resistance Amp
. Screen Zeo
. Strain Stbl3
. Culture temperature 37degrees centigrade
. Promoter: EF-1 alpha core promoter
. Copier: Ori, F1 ori, SV40 ori
. Terminator: WPRE
. Plasmid classification: mammalian cell plasmid; mammalian editing plasmid; mammalian Cas9 plasmid
. Plasmid size: 13177 BP
. Plasmid tags: C-nucleoplasmin NLS, C-FLAG, C-EGFP
. Prokaryotic resistance: ampicillin Amp
. Screening Marker: Bleomycin Zeo
. Cloning strain: Escherichia coli Stbl3
. Culture conditions: 37 C, aerobic, LB
. Expressing host: mammalian cells such as 293T
. LentiCas9-EGFP Description
. Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.
. LentiCas9-EGFP Reference
. Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.