MycoReal Kit Ascosphaera apis is a real-time PCR kit for detection of Ascosphaera apis DNA. This test was developed for the Applied Biosystems® 7500 (Fast) instrument (Thermo Fisher Scientific), LightCycler® 480 (Roche) and for Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of DNA of Ascosphaera apis from samples purified from diseased larvae and/or honey samples (spores) (e.g. with the QIAGEN DNeasy Plant Mini Kit). Cell/spore suspensions and suspensions containing only spores have to be differentiated. Suspensions containing only spores require a more complex DNA extraction step .