ViroReal® Kit Bluetongue Virus (BTV) is a real-time PCR kit for the detection of bluetongue virus (BTV) RNA using one-step reverse transcription real-time PCR. This test was developed for the ABI PRISM® 7500 (Fast) instrument (Thermo Fisher Scientific), LightCycler® 480 (Roche) and for Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This test allows the rapid and sensitive detection of BTV RNA purified from tissues (e.g. spleen, lymph nodes, etc.) and blood (e.g. with the QIAamp Viral RNA Mini Kit, Qiagen).