ViroReal® Kit TGEV is a real-time PCR assay for detection of RNA of the transmissible gastroenteritis
coronavirus (TGEV) using one-step reverse transcription real-time PCR. This test was developed and validated for the ABI PRISM® 7500 (Fast) instrument (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® (Agilent), but is also suitable for other real-time PCR instruments. This kit allows the rapid and sensitive detection of TGEV RNA isolated from nasal swabs and feces (e.g. with the QIAamp Viral RNA Mini Kit, Qiagen). The test does not detect PRCV which has a large in-frame deletion in the 5’ end of the S gene.